Major Employers in Mattoon–
| Employer | Location | Employees | Product/Service |
|---|---|---|---|
| Rural King | Mattoon | 433 | Corporate Headquarters is located in Mattoon. There are 113 Stores Nationwide with a total of 8,538 Employees |
| Consolidated Communications | Mattoon | 420 | Corporate Headquarters with locations in TX, PA, CA, and KS with a total of 1350 Employees |
| Sarah Bush Lincoln Health Center | Mattoon | 2450 | Health Care/ Hospital |
| Pinnacle Foods | Mattoon | 250 | Lenders Bagel Bakery |
| Justrite Manufacturing Co., Inc. | Mattoon | 185 | Safety Containers |
| Mars Pet Care, US | Mattoon | 150 | Pet Foods |
| LSC Communications | Mattoon | 968 | Communications Company |
| Mattoon Precision Mfg Co., Inc. | Mattoon | 145 | Automotive Parts |
| Blue Cross/Blue Shield | Mattoon | 125 | Regional Office / Headquarters |
| Anamet Electrical, Inc. | Mattoon | 118 | Metal & Plastic Flexible Conduit |
| United Graphics, L.L.C. | Mattoon | 116 | Printing |
| A.J. Walker Construction Co. | Mattoon | 100 | Redi-Mix Concrete |
| Ameren Services | Mattoon | 100 | Utility |
| Electric Laboratories & Sales | Mattoon | 64 | Electrical Supply Sales |
| Coles Moultire Electric Co-Op | Mattoon | 35 | Electric Co-Op |
| City of Mattoon | Mattoon | 200 | Municipality |
| Kingery Printing Company United Graphics Division | Mattoon | 130 | Printing and Graphics |
| UPS | Mattoon | 70 | Delivery service |
| Heartland Coca-Cola | Mattoon | 64 | Coca-Cola products |
| First Mid Bank & Trust | Mattoon | 730 | Financial institution |
| Walmart Supercenter | Mattoon | 360 | Discount department store |
| Ameren CIPS | Mattoon | 200 | Electric |
| Commercial Refrigeration of Central Illinois, Inc | Mattoon | 49 | Illinois Transport Company |
| Odd Fellow Rebekah Home | Mattoon | 135 | Skilled Nursing home short and long term care |
| Mattoon Community School District #2 | Mattoon | 525 | School District |
| Lake Land College | Mattoon | 1172 | Community College |
| Eastern Illinois University | Charleston | 1850 | University |